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CD278 (ICOS) Mouse anti-Human, PerCP-eFluor™ 710, Clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9948-42

Additional Details : Weight : 0.23750kg

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Product Code. 15559466

  • 2835.00 SEK / 100 tests


Estimated Shipment
02-12-2020
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Description

Description

Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered. CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.
Specifications

Specifications

CD278 (ICOS)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9Y6W8
AILIM, CD278, CVID1, activation-inducible lymphocyte immunomediatory molecule, inducible T-cell costimulator, inducible costimulator
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
ISA-3
PerCP-eFluor™ 710
ICOS
Liquid
ICOS
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
29851
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