missing translation for 'onlineSavingsMsg'
Learn More

CD3 Mouse anti-Human, eFluor™ 506, Clone: UCHT1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 69-0038-42

Additional Details : Weight : 0.23750kg

 View more versions of this product

Product Code. 15560637

  • 2215.00 SEK / 100 tests

Estimated Shipment: 27-07-2021

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.
Specifications

Specifications

CD3
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P20963, P09693, P07766, P04234
FLJ18683; T3E; TCRE; HCD3
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
UCHT1
eFluor™ 506
CD3E
Liquid
CD247, CD3D, CD3E, CD3G
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
915, 916, 917, 919
Videos
SDS
Documents
Certificates

For Research Use Only.