CD366 (TIM3) Rat anti-Mouse, Clone: 8B.2C12, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5871-81
Additional Details : Weight : 0.01000kg
DescriptionThe 8B.2C12 antibody binds to the BALB/c allele of CD366 while reactivity to the C57Bl/6 allele is significantly weaker. Applications Reported: The 8B.2C12 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported for use in functional assays. (Please use Functional Grade purified 8B.2C12, cat. 16-5871, in functional assays.). Applications Tested: The 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes and mouse CD366 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
|PBS with 0.09% sodium azide; pH 7.2|
|Flow Cytometry, Immunohistochemistry, Immunoprecipitation|
For Research Use Only.