CD4, clone: OX35, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0040-82
Additional Details : Weight : 0.01000kg
DescriptionDescription: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ double-positive and CD4+ single-positive thymocytes, and by CD4+ helper T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.Applications Reported: This OX35 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Flow CytometryþImmunohistochemistry (Frozen)|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.