Invitrogen™ CD4 Monoclonal Antibody (4SM95), eBioscience™, Invitrogen™

Description

Description: This 4SM95 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. The 4SM95 monoclonal antibody is not recommended for immunohistochemistry of frozen mouse tissue. For detection of mouse CD4 using immunohistochemistry of frozen tissue please refer to alternate products Anti-Mouse CD4 Purified (cat. 14-0041 and 14-0042). Applications Reported: This 4SM95 antibody has been reported for use in western blotting, microscopy, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 4SM95 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 μg/mL. This 4SM95 antibody has also been tested by western blot of reduced mouse spleen lysate and can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].