CD5, clone: 53-7.3, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0051-85
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: The 53-7.3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.Applications Tested: The 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1|
|Flow Cytometry, Immunohistochemistry, Immunoprecipitation|
|IgG2a, kappa, kappa|
|Store at 2-8°C.|
For Research Use Only.