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CD5, clone: 53-7.3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0051-85

Additional Details : Weight : 0.01000kg

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Product Code. 15236767

  • 1536.00 SEK / 500µg


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Description

Description

Description: The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67kDa protein expressed by a majority of thymocytes, mature T cells and a subset of B cells. The expression of CD5 by a small subset of B cells characterizes a developmentally and functionally distinct lineage of B cells called B-1 cells. CD5 is a counter-receptor for CD72 and plays a role in the T-B cell interaction.Applications Reported: The 53-7.3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.Applications Tested: The 53-7.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD5
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P13379
CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
53-7.3
Unconjugated
Cd5
Liquid
Cd5
IgG2a, kappa, kappa
500μg
Store at 2-8°C.
Primary
12507
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