Learn More
Invitrogen™ CD70 Monoclonal Antibody (FR70), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen™ 14-0701-82
Description
Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. FR70 has also been reported in blocking of CD70 in vitro . (Please use Functional Grade purified FR70, cat. 16-0701, in functional assays.). Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 ÎĽg per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 ÎĽm post-manufacturing filtered.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.
Specifications
CD70 | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
O55237 | |
Cd70 | |
Affinity chromatography | |
RUO | |
21948 | |
4°C | |
Liquid |
Flow Cytometry, Functional Assay, Immunoprecipitation, Neutralization | |
FR70 | |
Unconjugated | |
Cd70 | |
CD27 ligand; Cd27l; CD27-L; CD27LG; CD70; CD70 antigen; Cd70 molecule; Ki-24 antigen; surface antigen CD70; Tnfsf7; TNLG8A; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8A; tumor necrosis factor ligand superfamily member 7 | |
Rat | |
100 ÎĽg | |
Primary | |
Mouse | |
Antibody | |
IgG2b Îş |
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only.