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CD80 (B7-1) Mouse anti-Human, Functional Grade, Clone: 2D10.4, eBioscience™

Mouse Monoclonal Antibody

1010.00 SEK - 2950.00 SEK

Specifications

Antigen CD80 (B7-1)
Clone 2D10.4
Target Species Human
Conjugate Functional Grade
Isotype IgG1, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15208517
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Affymetrix eBioscience
16-0809-81
50 μg
1010.00 SEK
50µg
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Estimated Shipment
26-05-2021
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15218517
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Affymetrix eBioscience
16-0809-85
500 μg
2950.00 SEK
500µg
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Estimated Shipment
22-06-2021
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Description

Description

Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: The 2D10.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 2D10.4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specifications

Specifications

CD80 (B7-1)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
2D10.4
Functional Grade
Flow Cytometry, Functional Assay
Primary
Monoclonal
PBS with no preservative; pH 7.2
RUO
SDS

For Research Use Only.