Invitrogen™ CD90 (Thy-1) Monoclonal Antibody (YKIX337.217), PE, eBioscience™, Invitrogen™

Description

Description: This YKIX337.217 monoclonal antibody reacts with canine CD90, which is also known as Thy-1. This 25-37 kDa N-glycoylsated cell adhesion molecule is expressed on thymocytes, peripheral T cells, some B cells, monocytes, and granulocytes albeit at a lower level. CD90 is also expressed on cells in the bone marrow, brain, and kidney, as well as on mesenchymal stem cells. This monoclonal antibody has been reported to deplete cells in vitro and in vivo. Applications Reported: This YKIX337.217 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX337.217 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.