CD96 (TACTILE), PerCP-eFluor™ 710, clone: NK92.39, eBioscience™

Description

Description: The NK92.39.1 monoclonal antibody reacts with human CD96 also known as TACTILE (T cells-activated increased late expression). CD96 is a type I membrane protein of the Immunoglobulin Superfamily. CD96 promotes NK cell adhesion to cells expressing the polio virus receptor (CD155). Therefore CD96 is an additional receptor for CD155. After binding of PVR to CD96, the PVR can be transferred to the target cell and CD96 can be internalized with bound PVR. This internalization may lead to cell death of the NK cell. This phenomenon may be significant in tumor cells which express high levels of PVR. Furthermore expression of CD96 has been found in the majority of CD34(+)CD38(-) Acute Myeloid Leukemic cells.)The NK92.39.1 antibody can block binding of soluble CD155 to NK cell lines.Applications Reported: This NK92.39 antibody has been reported for use in flow cytometric analysis.Applications Tested: This NK92.39 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.