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CDK2/cyclin E1 Recombinant Human Protein

CDK2/cyclin E1 Recombinant Human Protein

Brand:  Invitrogen PV6296

Additional Details : Weight : 0.01000kg

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Product Code. 15448943

  • 10180.00 SEK / 100µg


Estimated Shipment date
from Supplier 02-02-2018
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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Description

Description

CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

  • Validated in LanthaScreen™ Kinase Binding Assay
  • Protocols available for simple use and optimized performance
  • Multiple pack sizes available for convenient use

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  • Specifications

    Specifications

    NP_001789.2, NP_001229.1
    Pharma and biopharma, protein assays and analysis, target and lead identification and validation
    CDK2/Cyclin E1 Recombinant Human Protein
    >95% by SDS-PAGE
    –68 to –85°C
    1 Each
    CDK2/Cyclin E1 Recombinant Human Protein
    Kinase assay
    345nmoles of phosphate transferred to histone H1 per minute per mg of total protein at 30°C
    GST Tag
    0.1mg/mL
    60.2/73.4kDa
    7.5
    100μg
    For research use only. Not for use in diagnostic procedures
    CDK2: p33 (CDK2) cyclinE1: CCNE1, CCNE
    CDK2/cyclin E1 was pre-diluted in enzyme dilution buffer and assayed in 5mM MOPS (pH 7.2), 2.5mM β-glycerol-phosphate, 5mM MgCl2, 0.4mM EDTA, 1mM EGTA, 0.05mM DTT, with 50μM ATP, trace [33P]-γ-ATP and 200μg/mL histone H1 for 15 min.s at 30°C
    CDK2/Cyclin E1 Recombinant Human Protein
    Human
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