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Invitrogen™ CellBlox™ Blocking Buffer, 100 Tests
Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells, which can result in higher background labeling. Also recommended for cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling.
Brand: Invitrogen™ B001T03F01
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Description
- Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells that can result in higher background labeling
- CellBlox™ Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling
- Recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling
- Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols
- Add 5 ÎĽL of buffer directly to a cell suspension containing 103 to 108 cells prior to the addition of an antibody, with 100 ÎĽL as a final staining volume
- Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 ÎĽL of buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 ÎĽL as a final staining volume
- Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction
- Compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains
- Can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution
- CellBlox Blocking Buffer is compatible with other blocking reagents such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer
- Buffer is not required when labeling antibody-capture beads
Certificates
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5 results found
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 3048998 | Certificate of Analysis | 20/09/2024 | B001T03F01 |
| 2789924 | Certificate of Analysis | 12/09/2023 | B001T03F01 |
| 2585870 | Certificate of Analysis | 09/02/2023 | B001T03F01 |
| 2513929 | Certificate of Analysis | 21/07/2022 | B001T03F01 |
| M131516 | Certificate of Analysis | 05/05/2022 | B001T03F01 |
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Invitrogen™ CellBlox™ Blocking Buffer, 100 Tests > 100 tests
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