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Molecular Probes™ ChromaTide™ Alexa Fluor™ 594-5-dUTP

ChromaTide™ Alexa Fluor™ 594-5-dUTP

Brand:  Molecular Probes™ C11400

7429.00 SEK valid until 2024-06-28
Use promo code "21689" to get your promotional price.



Additional Details : Weight : 0.01000kg

Product Code. 10143622

  • 7820.00 SEK / Each
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For Research Use Only. All usage must comply with product instructions.


Description

Description

Molecular Probes™ ChromaTide™ dye labeled dUTP, OBEA-dCTP, and UTP nucleotides can be used to synthesize labeled DNA probes without the need for hazardous and expensive radioisotope-labeled nucleotides. These nucleotides can be incorporated using standard molecular biology techniques; labeled probes can then be used in in situ hybridization, microarray, or blotting protocols. ChromaTide™ dye labeled nucleotides are available in different fluorescent colors to facilitate multicolor analysis.

ChromaTide™ Labeled Nucleotides Specifications:
  • Ex/Em of dye: Alexa Fluor™ 594-5-dUTP (590/615 nm)
  • Length of alkynylamino linker: 5 atoms

    Methods for Incorporating ChromaTide™ Nucleotides Into Probes
  • Nick translation
  • Random primer labeling
  • End-labeling with terminal deoxynucleotidyl transferase
  • Reverse transcription
  • PCR amplification

    See Methods for Enzymatic Incorporation of ChromaTide™ dUTPs for specific guidelines for each of these methods.

    Alexa Fluor™ and BODIPY™ Fluorescent Dyes Make Excellent Probes
    Probes made with labeled nucleotides can be used for multicolor techniques such as in situ hybridization and hybridization to arrays. Our proprietary BODIPY™ and Alexa Fluor™ dye conjugates are exceptionally bright, photostable, and essentially pH insensitive. The narrow emission profile of the BODIPY™ dyes helps ensure minimal spectral overlap. The Alexa Fluor™ dyes are highly water soluble, as are DNA probes made from them, making them the labels of choice for fluorescence in situ hybridization.

    Long Linkers Improve Performance
    The ChromaTide™ dUTP and UTP nucleotides are modified at the C-5 position of uridine via a unique alkynylamino linker, which provides a spacer between the nucleotide and the dye to reduce interactions between them. The number in the product name, e.g., the “12” in fluorescein-12-dUTP, indicates the net length of the spacer, in atoms. These spacers result in brighter conjugates and increased hapten accessibility for secondary detection reagents.

    For complete listing of our ChromaTide™ Reagents: Molecular Probes ChromaTide™ and aha labeled nucleotides—Table 8.5.
    For additional information on these labeling reagents, read Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

    For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
  • Specifications

    Specifications

    Store in freezer (-5 to -30°C) and protect from light.
    Dry Ice
    Alexa Fluor™ 594
    Alexa Fluor™, ChromaTide™
    Direct Labeling
    1 mM
    25 μL
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    For Research Use Only. Not for use in diagnostic procedures.