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Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method)
These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.
Brand: Invitrogen™ EEA024
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Description
The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method) is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, tissues, cells, cell culture supernatant, saliva, and urine sample types. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample.
The assay quantifies the ability of the sample to reduce ferric ions (Fe3+) to ferrous ions (Fe2+) using the ferric reducing antioxidant power (FRAP) method under acidic conditions. T-AOC can be measured using the absorbance value of the resulting blue solution at 593 nm.
Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results
Specifications
| 0.23–2 mmol Trolox Equiv./L | |
| Store at 2–8°C. |
|
| Cell Culture Supernatant, Plasma, Saliva, Serum, Tissue Homogenates, Urine, Whole Cells | |
| 3-5 min. | |
| 3.9 | |
| 5 μL | |
| Total Antioxidant Capacity (T-AOC) |
| 0.049 mmol/L | |
| Colorimetric | |
| Microplate Reader | |
| 8.1 | |
| 96 tests | |
| Dry Ice, 2–8°C | |
| 20 min. |
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Product Title
Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (FRAP method) > 96 tests
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