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SuperScript™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Brand: SuperScript™ 1178101K
This item is not returnable.
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Description
- Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments
- This fast, one-step mix is designed to work with a variety of instruments’ default protocols, including Applied Biosystems and non-Applied Biosystem (see universal version) qPCR platforms
- Fast-activating, antibody-mediated Platinum Taq DNA Polymerase for complete, rapid activation; provides high sensitivity and reproducibility
- The One-Step SuperScript III Reverse Transcriptase module is a unique formulation to ensure high yield and less PCR inhibition
- Flexible formats with separate or pre-mixed ROX are designed to work with default instrument protocols on a wide range of high-throughput fast instruments
- Contains heat-labile UDG which significantly reduces carryover contamination risk with excellent activity from room temperature to approximately 40°C
- Due to the thermostability of SuperScript III, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated
- Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4°C to 8°C
- Store at -20°C for long-term storage
Specifications
| The EXPRESS One-Step SuperScript™ qRT-PCR Universal kit contains 3 modules: 2X EXPRESS SuperScript™ Mix (5 × 1 mL), Express qPCR SuperMix Universal (5 × 5 mL), and ROX Reference Dye (5 × 500 μL). Store at -20°C for long-term storage. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at 4–8°C. |
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| Tube | |
| Taq DNA Polymerase | |
| 7500 Fast System, 7500 System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, BioRad CFX96™, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ | |
| qRT-PCR Kit | |
| 2500 Reactions |
| Primer-probe | |
| 1-step RT-qPCR | |
| Fast or Standard | |
| SuperScript™ | |
| 2500 Reactions | |
| RNA |
For Research Use Only. Not for use in diagnostic procedures.