missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), Biotin, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen™ 13-5902-82
This item is not returnable.
View return policy
Description
Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 ÎĽg per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 ÎĽm post-manufacturing filtered.
GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
Specifications
| GL7 | |
| Monoclonal | |
| 0.5 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| Rat | |
| 100 ÎĽg | |
| Primary | |
| 4°C, do not freeze | |
| Liquid |
| Flow Cytometry | |
| GL-7 (GL7) | |
| Biotin | |
| GL7 antigen; Ly77; Ly-77 | |
| Affinity chromatography | |
| RUO | |
| Human, Mouse | |
| Antibody | |
| IgM |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), Biotin, eBioscience™, Invitrogen™ > 100 μg; Biotin
For Research Use Only.
Spot an opportunity for improvement?Share a Content Correction