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Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen™ 13-5902-82

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Product Code. 15209929

  • 1584.00 SEK / 100µg

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Description

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 ÎĽg per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 ÎĽm post-manufacturing filtered.

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

GL7
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Rat
100 ÎĽg
Primary
4°C, do not freeze
Liquid
Flow Cytometry
GL-7 (GL7)
Biotin
GL7 antigen; Ly77; Ly-77
Affinity chromatography
RUO
Human, Mouse
Antibody
IgM
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Product Title
Invitrogen™ GL7 Monoclonal Antibody (GL-7 (GL7)), Biotin, eBioscience™, Invitrogen™ > 100 ÎĽg; Biotin

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