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Granzyme M Mouse anti-Human, PE, Clone: 4B2G4, Invitrogen™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-9774-42
Additional Details : Weight : 0.01000kg
Description
Description: Granzyme M is one of five granzyme serine proteases that have been identified in humans. These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates. Although not as well characterized as some of the other family members, Granzyme M has been found to exhibit some distinct functions. These include the non-cytotoxic inhibition of cytomegalovirus replication, induction of apoptosis in tumor cells, and contribution to the TLR4-induced inflammatory process. Applications Reported: This 4B2G4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 4B2G4 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to 'Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins' located at Flow Protocols. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells.
Specifications
Granzyme M | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P51124 | |
GZMM | |
Affinity chromatography | |
RUO | |
3004 | |
4°C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
4B2G4 | |
PE | |
GZMM | |
Granzyme M; granzyme M (lymphocyte met-ase 1); GZMM; Hu-Met-1; Lmet1; lymphoctye Met-ase 1; lymphocyte met-ase 1; MET1; Met-1 serine protease; Met-ase; MMET-1; Natural killer cell granular protease; RNK-Met-1 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |