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HIF-1 alpha, clone: ESEE122, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-9100-80

Additional Details : Weight : 0.01000kg

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Product Code. 15258147

  • 946.00 SEK / 25µg


Estimated Shipment date
from Supplier 27-11-2018
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Description

Description

Description: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the constitutively expressed beta form, also called the aryl hydrocarbon receptor nuclear translocator (ARNT). Both alpha and beta subunits of HIF-1 contain basic helix-loop-helix motifs and function to activate transcription of genes in response to reduced oxygen levels. Under normoxic conditions, HIF-1 alpha is rapidly degraded, while HIF-1 alpha expression is induced and degradation inhibited under hypoxic conditions. HIF-1 alpha can be found in the cytoplasm and/or in the nucleus under normoxic conditions, but translocates to the nucleus under hypoxic conditions. HIF-1 is upregulated in several cancer types and functions to control genes involved in angiogenesis, cell survival and T cell development. Recent studies have shown the importance of HIF-1 alpha in balancing Th17 and Treg development.This ESEE122 antibody has also been reported to cross-react with bovine, rat, and mouse hypoxia inducible factor-1 alpha.Applications Reported: This ESEE122 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.Applications Tested: This ESEE122 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta at less than or equal to 10 ug/mL using low pH antigen retrieval buffer. This ESEE122 antibody has been tested by immunocytochemistry on fixed and permeabilized Hela cells at less than or equal to 10 ug/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

HIF-1 alpha
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9XTA5, Q16665, O35800, Q61221
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Bovine, Human, Murine, Rat
Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
ESEE122
Unconjugated
HIF1A
Liquid
HIF1A
IgG1, kappa, kappa
25μg
Store at 2-8°C.
Primary
15251, 281814, 29560, 3091
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