Learn More
Invitrogen™ IgM Monoclonal Antibody (II/41), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen™ 14-5790-81
Description
Description: The II/41 monoclonal antibody reacts with the ÎĽ heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM. Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. Applications Tested: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 ÎĽg per test. A test is defined as the amount (ÎĽg) of antibody that will stain a cell sample in a final volume of 100 ÎĽL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 ÎĽm post-manufacturing filtered.
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
Specifications
IgM | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P01872 | |
Ighm | |
Affinity Chromatography | |
RUO | |
16019 | |
4°C | |
Liquid |
ELISA, Flow Cytometry, Immunohistochemistry (Frozen) | |
II/41 | |
Unconjugated | |
Ighm | |
AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH | |
Rat | |
50 ÎĽg | |
Primary | |
Mouse | |
Antibody | |
IgG2a Îş |
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
For Research Use Only.