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IL-1 beta, Functional Grade, clone: B122, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-7012-81

Additional Details : Weight : 0.01000kg

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Product Code. 15248737

  • 760.00 SEK / 50µg

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Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.Applications Reported: The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.Applications Tested: The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-1 beta bioactivity. The B122 antibody at 0.125 μg/mL has been found to inhibit by 50% the biological effects of 4.0 ng/mL mouse IL-1 beta in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section.The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1 beta) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.


IL-1 beta
PBS with no preservative; pH 7.2
P10749, Q63264
Interleukin-1 beta, Catabolin, IL1B
Armenian Hamster
Affinity chromatography
Murine, Rat
ELISA, Functional Assay, Neutralization
Functional Grade
Store at 2-8°C.
16176, 24494

For Research Use Only.