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Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), PE, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 12-5887-81
This item is not returnable.
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Description
Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha 4/ beta 7), which is a heterodimer of the the 154 kDa alpha 4 subunit and the 130 kDa beta 7 subunit. In mouse, the beta 7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells. LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin. Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion. Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This DATK32 (DATK-32) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
Specifications
| Integrin alpha 4 beta 7 (LPAM-1) | |
| Monoclonal | |
| 0.2 mg/mL | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| P26011, Q00651 | |
| Itga4, Itgb7 | |
| Affinity chromatography | |
| RUO | |
| 16401, 16421 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
| Flow Cytometry | |
| DATK32 (DATK-32) | |
| PE | |
| Itgb7 | |
| CD49 antigen-like family member D; CD49D; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin beta 7; integrin beta-7; Integrin beta-P; Itga4; Itga4B; Itgb7; LPAM subunit alpha; Ly69; lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA4 subunit alpha; VLA-4 subunit alpha | |
| Rat | |
| 50 μg | |
| Primary | |
| Mouse | |
| Antibody | |
| IgG2a κ |
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Product Title
Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), PE, eBioscience™, Invitrogen™ > 50 μg; PE
For Research Use Only.
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