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Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
Brand: Invitrogen™ 12339024
Includes: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes: AccuPrime™ Taq DNA Polymerase (100μL, at 2U/μL); 10X AccuPrime™ PCR Buffer I (500μL); 10X AccuPrime™ PCR Buffer II (500μL); 50mM Magnesium Chloride (500μL); 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200mM Tris-HCl (pH 8.4), 500mM KCl, 15mM MgCl2, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
Description
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
Platinum anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C.
AccuPrime Taq DNA Polymerase features
- Control mispriming at every cycle for single-band PCR products
- Minimal reaction optimization and primer set redesign
- Reaction assembly at room temperature
Applications
AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including:
- Multiplex PCR
- TOPO TA Cloning
- Allele-specific amplifications
- High-throughput screening
AccuPrime buffers
The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
Order Info
Shipping Condition: Approved for shipment on wet or dry ice
Specifications
| • AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL) • 10X AccuPrime PCR Buffer I (2 x 1.25 mL) • 10X AccuPrime PCR Buffer II (2 x 1.25 mL) • 50 mM Magnesium Chloride (2 x 1.25 mL) Store at -20°C. |
|
| Low | |
| AccuPrime Taq DNA Polymerase | |
| DNA Polymerase System | |
| Wet or Dry Ice | |
| 10X | |
| Built-In Hot Start | |
| 3'-A | |
| 5 kb or less |
| Primer-probe | |
| 1-step RT-qPCR, Multiplex qPCR | |
| Standard | |
| 1,000 reactions | |
| Hot-start PCR | |
| 2X | |
| 1000 Reactions | |
| Separate Components |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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