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Invitrogen™ AccuPrime™ Taq DNA Polymerase System
Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications
Brand: Invitrogen™ 12339024
11033.89 SEK valid until 2023-09-23
Use promo code "21498" to get your promotional price.
Additional Details : Weight : 0.05000kg
Description
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.
- Specificity: Control mispriming at every cycle to get single-band PCR products
- Ease of use: minimize reaction optimization and primer set redesign
- Convenience: conveniently assemble reactions at room temperature
- AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg++, and deoxyribonucleotide triphosphates at concentrations
- Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
- For small genomic DNA amplicons (≤200bp), cDNA, or plasmids
Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR
Specifications
| AccuPrime™ Taq DNA Polymerase is provided in a 1000 reaction kit. Each kit includes: • AccuPrime™ Taq DNA Polymerase (500 µL, at 2U/µL) • 10X AccuPrime™ PCR Buffer I* (2 × 1.25 mL) • 10X AccuPrime™ PCR Buffer II* (2 × 1.25 mL) • 50 mM Magnesium Chloride (500 µL) *10XAccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb). 10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. Store at -20°C. |
|
| Tube | |
| 1-step RT-qPCR, Multiplex qPCR | |
| Standard | |
| AccuPrime™ | |
| Approved for shipment on Wet or Dry Ice | |
| Cloning | |
| 2 X | |
| 1000 Reactions | |
| Separate Components | |
| DNA |
| Primer-probe | |
| Low | |
| AccuPrime Taq DNA Polymerase | |
| 5' - 3' | |
| DNA Polymerase System | |
| 1000 Reactions | |
| 10X | |
| Built-In Hot Start | |
| 3'-A | |
| 5 kb or less |
For Research Use Only. Not for use in diagnostic procedures.