Invitrogen™ AccuPrime™ Taq DNA Polymerase System

Description

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.

• Specificity: Control mispriming at every cycle to get single-band PCR products
• Ease of use: minimize reaction optimization and primer set redesign
• Convenience: conveniently assemble reactions at room temperature

• AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations
• Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
• For small genomic DNA amplicons (≤200bp), cDNA, or plasmids

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR