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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Brand:  Invitrogen™ C404052

3682.80 SEK valid until 2021-12-31
Use promo code "14807" to get your promotional price.



Additional Details : Weight : 0.02000kg

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Product Code. 10082692

  • 4185.00 SEK / 20 reactions
Estimated Shipment: 07-12-2021

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For Research Use Only. All usage must comply with product instructions.


Description

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

Specifications

Specifications

TOP10
Yes
Electrocompetent
Yes
Not Suitable for preparing unmethylated DNA
20 reactions
Dry Ice
No
> 1x10^10
One Shot
One Shot™
Yes
Not Suitable for cloning unstable DNA
Lacks F' Episome
May be used for plasmids > 20 kb
Not for ccdB vector propagation
Yes
E. coli
High Efficiency (> 10^9 cfu⁄µg)
Transformation, Cloning
Not High-throughput Compatible (Manual)
E. coil
Videos
SDS
Documents
Certificates
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.