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Invitrogen™ T7 RNA Polymerase

DNA-dependent RNA polymerase with high specificity for bacteriophage T7 promoter sequences

Brand:  Invitrogen™ 18033019

Additional Details : Weight : 0.01000kg

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Product Code. 10093062

  • 2440.00 SEK / Each
Estimated Shipment: 15-12-2021

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For Research Use Only. All usage must comply with product instructions.


Description

Description

Synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is available.

  • Application: Synthesis of labeled and unlabeled RNA transcripts.
  • Source: Purified from E. coli expressing the T7 RNA Polymerase gene on a plasmid
  • Performance and Quality Testing: 3' and 5' exodeoxyribonuclease, ribonuclease, and DNA nicking assays; performance in a transcription reaction
  • Unit Definition: One unit hydrolyzes 1nMol of ribonucleotide into acid-precipitable material in 1 hr at 37°C using a T7 transcription vector as template
  • Unit Reaction Conditions: 40mM Tris-HCl (pH 8.0), 25mM NaCl, 8mM MgCl2, 2mM spermidine-(HCl)3, 5mM DTT, 0.4mM ATP, 0.4mM CTP, 0.4mM GTP, 0.4mM UTP, 1μCi [3H]GTP, 1μg SphI-cut pT7L13, and enzyme in 50μL for 10 min. at 37°C

Cell Free Protein Expression for Functional Analysis, Cell Free Protein Expression for Structural Analysis, Cell-Free (in vitro) Expression, Gene Expression Analysis and Genotyping, In Vitro Transcription, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Specifications

No
2500 units
DNA Polymerase
T7 RNA Polymerase
Wet Ice
T7
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.