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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

5550.00 SEK - 9950.00 SEK

Specifications

Product Type Cloning Kit
Quantity 25 Reactions
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Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10053172
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Invitrogen™
K462040
50 Reactions
9950.00 SEK
Each
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Estimated Shipment: 11-02-2022
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10738414
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Invitrogen™
K462001
25 Reactions
5550.00 SEK
Each
View alternative products
Save Up to 
»
Estimated Shipment: 11-02-2022
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Description

Description

Uses a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

Specifications

Specifications

Cloning Kit
25 Reactions
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.