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Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli Product Code.: 10462862
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Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

Product Code. 10462862
50 Reactions, Each
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Quantity:
25 Reactions
50 Reactions
Unit Size:
Each
This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Product Code. 10462862

Brand: Invitrogen™ K452040

Genomics Powerhouse
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10782.50 SEK valid until 2025-08-24
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This item is not returnable. View return policy

For Research Use Only. All usage must comply with product instructions.

Description

Includes

Topoisomerase I-activated pCR™2.1-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

They use a pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants.

  • 3'-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR2.1-TOPO) or T7 and SP6 (pCRII-TOPO) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
  • EcoR I sites flanking the PCR product insertion site for easy excision of inserts
  • Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
  • Easy blue/white colony screening for selection of recombinants

Cloning, PCR Cloning

TRUSTED_SUSTAINABILITY

Specifications

Bacterial or Yeast Strain DH5α
Cloning Method TOPO™-TA
Content And Storage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
Product Line One Shot™
Product Type Cloning Kit
Promoter T7, SP6
Quantity 50 Reactions
Vector TOPO-TA Cloning Vectors

Promotions

For Research Use Only. Not for use in diagnostic procedures.

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