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Invitrogen™ Jump-In™ Fast Gateway™ Core Kit

Jump-In™ Fast Gateway™ Core Kit

Brand: Invitrogen™ A10894

Product Code. 10587364

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Description

The Jump-In™ Fast Gateway™ Core Kit is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ Core Kit contains the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
Please note: the pJTI™ Fast DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway™ Pro Kits.
This kit is also a component of the Jump-In™ Fast Gateway™ System.

Specifications

Content And Storage	The Jump-In™ Fast Gateway™ Core Kit is composed of 2 vectors: pJTI™ Fast DEST Vector (150 ng⁄μl; total 40 μl), pJTI™ PhiC31 Int Vector (0.5 μg⁄μl; total 20 μl). Store vectors at -20°C.
Delivery Type	Transfection
Format	Kit
Vector	Jump-In Vectors, pJTI
For Use With (Application)	Transfection
Key Functions	Stable Cell Line Development, Targeted Integration, Clone your own Promoter
Product Type	Core Kit
Quantity	1 Kit
Selection Marker Eukaryotic	HygroR

Cloning Method	Gateway™
Cell Type	Mammalian
Promoter	None (Promoterless)
Expression System	Mammalian
High-throughput Compatibility	High-throughput Compatible
Product Line	Jump-In™
Protein Tag	Untagged
Selection Agent (Eukaryotic)	Hygromycin

Product Suggestions Documents

Documents

Product Information

Manuals

Jump-In Fast Gateway Targeted Integration System Version: 24 April 2012 File size: 910.2 kB

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