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Ly-49H Mouse anti-Mouse, Biotin, Clone: 3D10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-5886-82

Additional Details : Weight : 0.25000kg

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Product Code. 15219899

  • 1284.00 SEK / 100µg

Estimated Shipment: 08-09-2021

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Description

Description

Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells. Natural killer cells can express several Ly-49 proteins. It has been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783). Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice. Other mice strains have not been tested. No crossreactivity to Ly-49A, C, D or G2 has been observed. Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells. Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.

2 μm post- manufacturing filtered. Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells. Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors. Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells. Natural killer cells can express several Ly49 proteins.
Specifications

Specifications

Ly-49H
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60682
Klra8
IgG1, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16639
Flow Cytometry
3D10
Biotin
Klra8
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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