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Invitrogen™ Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand: Invitrogen™ 11-5895-85

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Product Code. 15218289

3115.00 SEK / 500µg

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Description

Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice. Applications Reported: YLI-90 has been reported for use in flow cytometric analysis. Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.

Specifications

Antigen	Ly-49I
Classification	Monoclonal
Concentration	0.5 mg/mL
Formulation	PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.	0
Gene Symbols	Klra9
Purification Method	Affinity chromatography
Regulatory Status	RUO
Gene ID (Entrez)	16640
Content And Storage	4°C, store in dark, DO NOT FREEZE!
Form	Liquid

Applications	Flow Cytometry
Clone	YLI-90
Conjugate	FITC
Gene	Klra9
Gene Alias	5E6; Killer cell lectin-like receptor 3; killer cell lectin-like receptor subfamily A, member 9; Klra3; Klra9; Ly-49; Ly49C; Ly-49c; Ly49I; Ly49I receptor; Ly-49I receptor; LY49I1; Lymphocyte antigen 49c; Nk2.1; T-cell surface glycoprotein Ly-49C
Host Species	Mouse
Quantity	500 μg
Primary or Secondary	Primary
Target Species	Mouse
Product Type	Antibody
Isotype	IgG1 κ

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Invitrogen™ Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™, Invitrogen™ > 500 μg; FITC

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Invitrogen™ Ly-49I Monoclonal Antibody (YLI-90), FITC, eBioscience™, Invitrogen™

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