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Ly-49I Mouse anti-Mouse, FITC, Clone: YLI-90, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-5895-85

Additional Details : Weight : 0.25000kg

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Product Code. 15218289

  • 2410.00 SEK / 500µg

Estimated Shipment: 26-07-2021

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Description

Description

Description: The YLI-90 monoclonal antibody reacts with the B6 mouse Ly-49I receptor. Ly49I, a member of the Ly49 family with inhibitory function, is a homodimer type II transmembrane protein and contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM). YLI-90 binds to subpopulations of NK, NKT and T cell lineages in the C57Bl/6 strain. This antibody does not bind to cells from BALB/c, SJL or AKR/J mice. Applications Reported: YLI-90 has been reported for use in flow cytometric analysis. Applications Tested: The YLI-90 antibody has been tested by flow cytometric analysis of C57Bl/6 and BALB/c mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
Specifications

Specifications

Ly-49I
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Liquid
Klra9
IgG1, kappa
500 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16640
Flow Cytometry
YLI-90
FITC
Klra9
Ly49I; Ly-49I receptor; Ly49I receptor
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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