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Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

Save time and to reduce the possibility of pipetting errors with the pre-mixed components in this complete system for efficient synthesis of first strand cDNA.

3110.00 SEK - 9750.00 SEK


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Thermo Scientific™
K1641
50 reactions
3110.00 SEK
50 reactions
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10334500
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Thermo Scientific™
K1642
200 reactions
9750.00 SEK
200 reactions
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Description

Description

Maxima First Strand cDNA Synthesis Kit for RT-qPCR is a convenient system optimized for cDNA synthesis in 2-step quantitative RT-PCR (RT-qPCR) applications. The kit uses Maxima Reverse Transcriptase (RT), an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features high thermostability, robustness and increased cDNA synthesis rate compared to wild type M-MuLV RT.

The Maxima First Strand cDNA Synthesis Kit for RT-qPCR is capable of reproducible cDNA synthesis from a wide range of total RNA amounts (1 pg to 5 µg) at elevated temperatures (42 to 65°C).The synthesis reaction can be completed in 15 to 30 minutes. Components of the Maxima First Strand cDNA Synthesis Kit for RT-qPCR are pre-mixed to save time and to reduce the possibility of pipetting errors.

The new Maxima First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.

Highlights

  • Integrated gDNA removal step
  • High yields of full length cDNA up to 20 kb.
  • Efficient cDNA synthesis at wide temperature range from 42°C to 65°C.
  • Increased synthesis rate - complete cDNA synthesis in 15-30 minutes.
  • High sensitivity and specificity.

Maxima Universal First Strand cDNA Synthesis Kit

Only available in US and Canada

The Maxima Universal First Strand cDNA Synthesis Kit differs from the Maxima First Strand cDNA Synthesis Kit by having separate kit components: Maxima Reverse Transcriptase, RiboLock RNase Inhibitor, 5X RT Buffer, 10 mM dNTPs, oligo (dT)18 primer, random hexamer primers, and nuclease-free water.

Maxima Universal First Strand cDNA Synthesis Kit

Only available in US and Canada

The Maxima Universal First Strand cDNA Synthesis Kit differs from the Maxima First Strand cDNA Synthesis Kit by having separate kit components: Maxima Reverse Transcriptase, RiboLock RNase Inhibitor, 5X RT Buffer, 10 mM dNTPs, oligo (dT)18 primer, random hexamer primers, and nuclease-free water.

SDS
Product Certifications

This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.