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Invitrogen™ Click-IT™ O-GlcNAc Enzymatic Labeling System
GREENER_CHOICE

For in-vitro modification of O-GlcNAc modified proteins

Brand:  Invitrogen™ C33368

Product Code. 10006892

  • 5420.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

This item is not returnable. View return policy

Description

Description

The Click-iT O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.

  • Less hazardous

Cell Analysis, Cell Structure, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Mitochondria, Mitochondria Related Diseases, Mitochondrial Pathways, Protein and Antibody Labeling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Less hazardous
1 Kit
Biotin-based, Fluorescence
Click-iT™
Proteins
O-GlcNAc Enzymatic Labeling System
Store in refrigerator at 2°C to 8°C
Wet Ice
Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, Biotin, Oregon Green 488, TMR (Tetramethylrhodamine)
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For Research Use Only. Not for use in diagnostic procedures.