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Invitrogen™ TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit, with Mammalian TC-Tag Gateway™ Expression Vectors (Green Fluorescence) (Red Fluorescence)

An expression tag-based fluorescence labeling system for live-cell labeling

Brand:  Invitrogen™ T34563

Product Code. 10616203

  • 20810.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.


Includes: The kit contains FlAsH-EDT2 labeling reagent, ReAsH-EDT2 labeling reagent, BAL wash buffer, pcDNA6.2/cTC-Tag-DEST, pcDNA6.2/nTC-Tag-DEST, and pcDNA6.2/nTC-Tag-p64 Control Plasmid.
This item is not returnable. View return policy

Description

Description

The expression construct is created when the Gateway™ entry clone (containing the gene of interest) is recombined with one of two destination vectors (pcDNA6.2/nTC-Tag-DEST for N-terminal tagging or pcDNA6.2/cTC-Tag-DEST for C-terminal tagging). The expression construct is then used to transform the host strain. The protein can be detected either with green-fluorescent FlAsH-EDT2 reagent or red-fluorescent ReAsH-EDT2 reagent. The tag is very small (6 amino acids) and the protein of interest is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Enzyme and Protein Activity Assays, Labeling Chemistry, Lumio Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays, Tetracysteine (Lumio/FlAsH/ReAsH) Assays

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Tetracysteine Tag Detection Kit
Fluorescence Microscope
Store in freezer (-5 to -30°C) and protect from light.
FlAsH, ReAsH
Kit
1 Kit
Fluorescence
Tetracysteine Tag Detection
Red, Green
Wet Ice
Gateway, TC-FlAsH II, TC-ReAsH II
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5 results found
Lot Number Certificate Type Date Catalog Number
Lot Number3008215 Certificate TypeCertificate of Analysis Date03/09/2024 Catalog NumberT34563
Lot Number2826930 Certificate TypeCertificate of Analysis Date06/11/2023 Catalog NumberT34563
Lot Number2770777 Certificate TypeCertificate of Analysis Date28/09/2023 Catalog NumberT34563
Lot Number2539053 Certificate TypeCertificate of Analysis Date16/02/2023 Catalog NumberT34563
Lot Number2507856 Certificate TypeCertificate of Analysis Date19/07/2022 Catalog NumberT34563
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Product Title
Invitrogen™ TC-FlAsH™ TC-ReAsH™ II In-Cell Tetracysteine Tag Detection Kit, with Mammalian TC-Tag Gateway™ Expression Vectors (Green Fluorescence) (Red Fluorescence) > 1 kit

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For Research Use Only. Not for use in diagnostic procedures.