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Gibco™ Mouse IL-3 Recombinant Protein

Recombinant Protein

Brand: Gibco™ PMC0031

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Product Code. 10079072

20380.00 SEK / Each

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For Research Use Only. All usage must comply with product instructions.

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Description

Carrier-Free Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in deionized water to 0.1-1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.

Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Specifications

Accession Number	P01586
Formulation	protein with no preservative
Molecular Weight (g/mol)	16.1 kDa
Purification Method	Sequential Chromatography
Endotoxin Concentration	<0.1 EU/ μg
Common Name	IL-3
Conjugate	Unconjugated
Recombinant	Recombinant
Content And Storage	4°C
Endotoxin Level	<0.1 ng/μg
Expression System	E. coli
Protein Form	Recombinant, Ligand
Protein Subtype	Interleukins
Product Line	Gibco™

For Use With (Application)	Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Gene ID (Entrez)	16187
Name	Mouse IL-3
Quantity	100 μg
Gene Alias	BP/BPA; BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Csfmu; Eo-CSF; HCGF; hematopoietic growth factor; Il3; Il-3; ILN; Interleukin; interleukin 3; Interleukin 3 (colony stimulating factor; Interleukin3; interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; MULTI-CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA; PSF
Biological Activity	ED50 < 0.1 ng/mL; determined by the dose-dependent proliferation of NFS-60 cells.
Species	Mouse
Sequence	ASISGRDTHR LTRTLNCSSI VKEIIGKLPE PELKTDDEGP SLRNKSFRRV NLSKFVESQG EVDPEDRYVI KSNLQKLNCC LPTSANDSAL PGVFIRDLDD FRKKLRFYMV HLNDLETVLT SRPPQPASGS VSPNRGTVEC
Classification	Carrier-Free
Shipping Condition	Wet Ice
Protein Family	Cytokines & Receptors
Form	Lyophilized
Research Category	Immunology
Purity or Quality Grade	>95% by SDS-PAGE

Product Suggestions Documents

Documents

Product Information

Manuals

Product Sheet: Recombinant Mouse Interleukin-3 (IL-3) Version: B.0 File size: 57.5 kB

Certificate of Origin_PMC0031, PMC0033, PMC0034, PMC0035 (Mouse IL-3) Version: 0.0 File size: 28.8 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2509770	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberPMC0031
Lot Number2715960	Certificate TypeCertificate of Analysis	Date28/01/2025	Catalog NumberPMC0031
Lot Number1687201C	Certificate TypeCertificate of Analysis	Date16/04/2017	Catalog NumberPMC0031
Lot Number489815M	Certificate TypeCertificate of Analysis	Date14/04/2017	Catalog NumberPMC0031
Lot Number1640833C	Certificate TypeCertificate of Analysis	Date13/04/2017	Catalog NumberPMC0031

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