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Macherey-Nagel™ NucleoSpin™ DNA Stool

Combines enzymatic lysis with mechanical disruption of cell walls for efficient isolation of high-quality genomic DNA from any stool sample, fresh or frozen. Complete kit includes NucleoSpin™ Bead Tubes.

Brand:  Macherey-Nagel™ 740472.50

Additional Details : Weight : 0.45000kg Transport : UN number : 1993 Chem class : 3 Pack group : III


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Product Code. 15573626

  • 2873.00 SEK / 50 reactions


Estimated Shipment
15-12-2020
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Description

Description

Isolation of genomic DNA from bacterial and epithelial cells from stool samples

  • Efficient procedure combining enzymatic lysis and bead disruption
  • Isolation of high-quality genomic DNA (microbial and host) from fresh and frozen samples
  • Suitable for any stool sample – carnivores, omnivores, and herbivore
  • Bead Tubes included for efficient lysis – compatible with most common disruption devices
  • NucleoSpin™ Inhibitor Removal Columns ensure highest purity of the isolated DNA
  • Downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
Specifications

Specifications

Isolation of genomic DNA from stool samples
fresh or frozen stool samples from herbivores, omnivores, or carnivores
Mini Spin Column
Silica membrane and mechanical (bead tube) disruption
Approx. 200 mg (180 - 220 mg)
60 min/10 prep
Varies by sample type, quality and water content
Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer
Binding capacity: 50 μg
DNA Stool Columns, Bead Tubes Type A, Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), Buffers
50
Room temperature (18 - 25 °C)
Elution volume: 30 - 100 μL
Fragment size: 200 bp - approx. 50 kbp
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Safety and Handling

Safety and Handling

Product Identifier
  • NucleoSpin DNA Stool

Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.
  • H412-Harmful to aquatic life with long lasting effects.
  • EUH032-Contact with acids liberates very toxic gas.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash with water thoroughly after handling.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P330-Rinse mouth.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.

SDS
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