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Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification Product Code.: 11962262
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Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification

Product Code. 11962262
250rxns, 250 reactions
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Sufficient For:
10rxns
250rxns
Unit Size:
10 reactions
250 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 11962262

Brand: Macherey-Nagel™ 740966.250

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This item is not returnable. View return policy

Parallel isolation of RNA, DNA and, protein from an undivided sample.

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT

  • Silica membrane technology
  • Format: minispin columns
  • Sample size: <107 cultured cells, <30mg human/tissue; <50 mg plant tissue, <150ÎĽL reaction mixture
  • Fragment size, small RNA: 18 to 200 bases
  • Fragment size, large RNA: >200 bases
  • Typical yield: 10ÎĽg small RNA; 95ÎĽg large RNA from 107 HeLa cells
  • Binding capacity: 200ÎĽg
  • Elution volume, 30ÎĽL to 100ÎĽL
  • Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
  • Small RNA (18 to 200 bases)
  • Large RNA (>200 bases)
  • Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Specifications

Product Type NucleoSpin™ TriPrep Column Set
Quantity 1 Set
Includes 2mL Collection Tubes, 1.5mL Collection Tubes, NucleoSpin™ Filters, buffers, RNase-free and rDNase
Sufficient For 250rxns
Column Type Spin Column
For Use With (Application) For Rapid Purification of Total RNA, DNA, and Protein From Small and Precious Samples
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Product Identifier
  • NucleoSpin TriPrep (250)
Signal Word
  • Danger
Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2
Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P273-Avoid release to the environment.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
Supplemental information
  • MIXTURE LIST-Contains : zinc chloride, guanidium thiocyanate, ethanol
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