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OPTIZYME™ Alkaline Phosphatase, Fisher BioReagents™
2255.00 SEK - 3005.00 SEK
Specifications
| Concentration | 1 U/μL |
|---|---|
| Color | Colorless |
| pH | 7.4 |
| For Use With (Application) | DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En |
| Storage Buffer | OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
12867142
|
Fisher Bioreagents
BP8097-1 |
1000 U |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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|
12897142
|
Fisher Bioreagents
BP8097-5 |
3000 U |
This item is currently unavailable or has been discontinued. View the product page for possible alternatives. |
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Description
OPTIZYME™ AP is a novel alkaline phosphatase that catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides in 10min.
Applications and Features:
- OPTIZYME™ Alkaline Phosphatase dephosphorylates cloning vector DNA (10min at 37°C)
- Fast thermoinactivation (5min at 75°C)
- Simultaneous digestion and dephosphorylation of vector DNA
- Single protocol for all types of DNA ends
- Nucleotide degradation prior to sequencing PCR products
- Active on nucleotides
- Protein dephosphorylation
- Active in all OPTIZYME restriction enzyme buffers
Supplied with:
10X OPTIZYME AP Buffer: 100mM Tris-HCl (pH 8.0 at 37°C), 50mM MgCl2, 1M KCl, 0.2% (v/v) Triton X-100, 1mg/mL BSA
Source: E. Coli cells with a cloned bacterial alkaline phosphatase gene
Quality Control:
- Endo-, exodeoxyribonuclease and ribonuclease-free
- Functionally tested for dephosphorylation of 5'-termini of overhanging, recessed and blunt DNA
Specifications
| 1 U/μL | |
| 7.4 | |
| OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL | |
| E. coli cells with a cloned bacterial Alkaline Phosphatase gene | |
| Solid |
| Colorless | |
| DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En | |
| -20°C | |
| Alkaline Phosphatase |