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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

1228.00 SEK - 3035.00 SEK

Specifications

Antigen OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Clone eBio25-D1.16 (25-D1.16)
Concentration 0.5 mg/mL
Applications Flow Cytometry, Immunohistochemistry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15227447
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Affymetrix eBioscience
14-5743-81
50 μg
1228.00 SEK
50µg
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Estimated Shipment: 08-05-2024
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15237447
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Affymetrix eBioscience
14-5743-82
100 μg
3035.00 SEK
100µg
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Description

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM. 2. Incubate cells at 37°C for 2 hours. 3. Wash cells with flow staining buffer. 4. Proceed with cell surface staining as normal. For additional information see the references listed below. Purity: Greater than 90%, as determined by SDS-PAGE.

OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples. It is validated for Flow Cytometry, Immunohistochemistry.
Specifications

Specifications

OVA257-264 (SIINFEKL) peptide bound to H-2Kb
0.5 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
IgG1 κ
Affinity chromatography
Antibody
eBio25-D1.16 (25-D1.16)
Flow Cytometry, Immunohistochemistry
Unconjugated
Mouse
Mouse
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Primary
4° C
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.