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OVA257-264 (SIINFEKL) peptide bound to H-2Kb Mouse anti-Mouse, Clone: eBio25-D1.16 (25-D1.16), eBioscience™

Mouse Monoclonal Antibody

1016.00 SEK - 2520.00 SEK

Specifications

Antigen OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Clone eBio25-D1.16 (25-D1.16)
Target Species Mouse
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15227447
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Affymetrix eBioscience
14-5743-81
50 μg
1016.00 SEK
50µg
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Estimated Shipment: 09-09-2021
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15237447
View Documents
Affymetrix eBioscience
14-5743-82
100 μg
2520.00 SEK
100µg
View alternative products
Save Up to 
»
Estimated Shipment: 09-09-2021
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide. This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo. Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1. With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM. 2. Incubate cells at 37°C for 2 hours. 3. Wash cells with flow staining buffer. 4. Proceed with cell surface staining as normal. For additional information see the references listed below. Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
Specifications

Specifications

OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Mouse
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
eBio25-D1.16 (25-D1.16)
Unconjugated
Flow Cytometry, Immunohistochemistry
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS
Product Certifications

For Research Use Only.