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Phospho-ZAP70/Syk (Tyr319, Tyr352) Mouse anti-Human, Mouse, PE, Clone: n3kobu5, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-9006-42

Additional Details : Weight : 0.23750kg

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Product Code. 15557146

  • 2765.00 SEK / 100 tests


In stock, ready to ship: 5
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Description

Description

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70/SYK Y319/Y352 are located in the so-called interdomain of ZAP-70/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain). Phosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.
Specifications

Specifications

Phospho-ZAP70/Syk (Tyr319, Tyr352)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P48025, P43404, P43405, P43403
SYK, Zap70
IgG2b, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
20963, 22637, 6850, 7535
Flow Cytometry
n3kobu5
PE
SYK
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
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