Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA
Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.
Brand: Thermo Scientific™ 89804
Additional Details : Weight : 0.10000kg Transport : UN number : 3261 Chem class : 8 Pack group : III
DescriptionThese two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples. ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N-Acetyl glucosamine (GlcNAc) groups and to sialic acid. The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns. The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.
Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.
High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers
Fast – glycoprotein purification in less than one hour
Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate
Robust – lectin does not leach from resin when processing sample
Convenient – complete kits include lectin resins and spin columns with all necessary reagents
Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay
ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.
|Agarose Resin, Affinity|
|Kit, Microcentrifuge, Spin Column|
|Pierce Glycoprotein Isolation Kit, ConA|
|Sufficient For: Isolating glycoproteins with strong affinity for ConA from 10 samples of up to 640 µL (1 to 1.5 mg total protein) each
• ConA Lectin Resin, 1.1 mL
• Binding/Wash Buffer (5X), 6.5 mL
• Elution Buffer, 5 mL
• Spin Columns, 10 columns
• Collection Tubes, 20 tubes
Store at 4°C.