Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, ConA

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

Highlights:

• High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers

• Fast – glycoprotein purification in less than one hour

• Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate

• Robust – lectin does not leach from resin when processing sample

• Convenient – complete kits include lectin resins and spin columns with all necessary reagents

• Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Includes:

ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.