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Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit
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The Thermo Scientific™ Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than two hours) in the presence of firefly luciferase reporters for protein expression assays.
Highlights:
Sensitive – highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer
Stable – increased signal stability compared to flash assays
Simple – does not require a luminometer with injectors
Compatible – assay reagents compatible with other firefly beetle luciferases
Automation-friendly – amenable to high throughput assays
Convenient – contains a universal cell lysis buffer and optimized glow assay reagent
Safe – allows one to perform non-radioactive assays
Includes:
Cell lysis buffer, reaction buffer and substrate
Requires:
Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; For best results (maximum signal), use with Thermo Scientific Pierce Firefly Luciferase Signal Enhancer
Recommended for:
Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications
Specifications
Type | Assay Kit |
Target | Luciferase, Firefly Luciferase |
Quantity | 100 Reaction Kit |
Content And Storage | • Firefly Glow Assay Buffer, 5 mL, store at -20°C • D-Luciferin, Lyophilized, 3 mg, store at 4°C • 2X Cell Lysis Buffer, 6 mL, store at room temperature Upon receipt store kit at -20°C or store individual components as indicated above. |
Assay | Reporter Enzyme, Luciferase Reporter Assay |
Compatible Cells | Mammalian Cells |
Detection Method | Bioluminescence |
For Use With (Equipment) | Luminometer (Microplate) |
Format | 384-well plate, 96-well plate |
Label Type | Enzyme Labeled |
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For Research Use Only. Not for use in diagnostic procedures.
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