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Thermo Scientific™ Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible Product Code.: 17173259

Thermo Scientific™ Pierce™ High Capacity Ni-IMAC Resin, EDTA compatible

Product Code. p-7089908 Shop All Thermo Scientific Products
10 mL, 10mL
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Product Code. 17173259

Brand: Thermo Scientific™ A50584

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This item is not returnable. View return policy

Pierce high-capacity EDTA-compatible Ni-IMAC resin is designed for the purification of over-expressed His-tagged proteins from cell culture media.

Pierce high capacity Ni-IMAC resin contains a matrix that is a 6% crosslinked agarose with optimal properties for batch, gravity column, and research/pilot scale FPLC column purifications. The resin has high binding capacity (up to 80 mg of protein per mL of resin) and can tolerate the high EDTA and reducing agent concentrations used to inhibit metalloproteases and stabilize sensitive intracellular proteins.

Features of the high-capacity EDTA-compatible Ni-IMAC resin include:

  • Chelator stable—use up to 20 mM EDTA and DTT with no loss in performance
  • Compatible with purification of overexpressed, secreted proteins in cell culture media (e.g., Expi293, ExpiCHO, and ExpiSf expression systems)
  • High binding capacity—>80 mg/mL binding capacity for His-tagged recombinant proteins
  • Reusable—can be regenerated up to 10 times using the CIP protocol

EDTA and reducing agents such as DTT and TCEP adversely affect the performance of Ni-IMAC supports by stripping off the metal. Because of its chelator stability characteristic (EDTA tolerant), Pierce high-capacity EDTA-compatible Ni-IMAC resin is especially suited for purifying expressed His-tagged proteins that are secreted into cell culture media or for purifying intracellular His-tagged proteins that need the presence of EDTA to maintain stability and function. In both cases, large quantities of samples can be purified without the Ni metal stripping off of the support, thereby providing consistent high capacity yields.

TRUSTED_SUSTAINABILITY

Specifications

Capacity (Metric) 80 mg per mL Binding
Binding Property Chelator Stable
Matrix 6% crosslinked agarose
Content And Storage 10 mL settled resin, supplied as 20 mL 50% slurry. Store at 2–8°C.
Shipping Condition Wet Ice
Particle Size 50 to 80 ÎĽm
Form Liquid Suspension
Product Line Pierce™
Quantity 10 mL
Type Resin
Column Type Bottle
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5 results found
Lot Number Certificate Type Date Product Code
Lot NumberZL4573061 Certificate TypeCertificate of Analysis Date31/01/2025 Product CodeA50584
Lot NumberZK4493931 Certificate TypeCertificate of Analysis Date05/11/2024 Product CodeA50584
Lot NumberZI4404302 Certificate TypeCertificate of Analysis Date12/09/2024 Product CodeA50584
Lot NumberZG4379461 Certificate TypeCertificate of Analysis Date08/08/2024 Product CodeA50584
Lot NumberZF4335531 Certificate TypeCertificate of Analysis Date22/06/2024 Product CodeA50584

For Research Use Only. Not for use in diagnostic procedures.

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