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Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit
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Thermo Scientific™ Pierce Mouse IgG1; Fab and F(ab')2 Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments, depending on the chosen reaction conditions.
Highlights:
Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease
Optimized for mouse IgG1 – the kit procedure is specifically optimized for monoclonal mouse IgG1 antibodies, providing the best possible yield for this antibody subclass
High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time. Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG
Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments
Includes:
Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
Specifications
Specifications
| Quantity | 2.5 mL kit |
| Product Line | Pierce™ |
| Content And Storage | Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG • Pierce Ficin Agarose, 2.5 mL • Cysteine-HCl, 1 g • Mouse IgG1 Digestion Buffer, 120 mL • NAb Protein A Plus Spin Column, 1 mL, 1 column • Protein A Binding Buffer, 120 mL • IgG Elution Buffer, 120 mL • Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns • Spin Columns, 0.8 mL, 10 columns • Microcentrifuge tubes, 2 mL, 30 tubes Store at 4–8°C. |
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Safety and Handling
- Pierce Mouse IgG1 Fab and F(ab')2 Preparation Kit
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : Sodium chloride, L-(+)-Cysteine hydrochloride monohydrate
For Research Use Only. Not for use in diagnostic procedures.
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