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Thermo Scientific™ Sulfo-SDAD (Sulfo-NHS-SS-Diazirine) (sulfosuccinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Brand: Thermo Scientific™ 26175
Additional Details : CAS Number : 342405-30-5 Weight : 1.00000kg
Description
Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.
The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.Highlights:
Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions
Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps
Reversible - disulfide-containing spacers enable cleavage using reducing agents
Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light
Specifications
Amine-Nonselective | |
Sulfo-SDAD | |
No | |
Upon receipt store product at 4°C protected from light and moisture. | |
Ambient | |
Chemical Labeling | |
Diazirine, Sulfo-NHS Ester | |
Solid | |
Water | |
Yes |
By Thiols | |
490.51 | |
13.5 Å | |
No | |
Pierce™ | |
Heterobifunctional | |
Medium (10 to 30 Å) | |
50 mg | |
Standard | |
Crosslinker |
For Research Use Only. Not for use in diagnostic procedures.