missing translation for 'onlineSavingsMsg'
Learn More

T-bet Mouse anti-Human, Mouse, Rhesus Monkey, Alexa Fluor™ 488, Clone: eBio4B10 (4B10), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-5825-82

Additional Details : Weight : 0.00100kg

 View more versions of this product

Product Code. 16617825

  • 3900.00 SEK / 100µg
Estimated Shipment: 02-09-2022
to see stock.

Please to purchase this item. Need a web account? Register with us today!


Description

Description

Description: The eBio4B10 monoclonal antibody reacts with mouse, rhesus moneky and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells. Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This eBio4B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product No. 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at thermofisher.com/flowprotocols. This may be used at less than or equal to 0.25 μg per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
Specifications

Specifications

T-bet
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q9JKD8, Q9UL17
Mouse
100 μg
Primary
Rhesus Monkey, Mouse, Human
Antibody
IgG1 κ
Flow Cytometry
eBio4B10 (4B10)
Alexa Fluor 488
TBX21
TBX21
Affinity Chromatography
RUO
30009, 57765, 694044
4°C, store in dark, DO NOT FREEZE!
Liquid
Videos
SDS
Documents
Certificates
Special Offers

Special Offers