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Molecular Probes™ Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
Brand: Molecular Probes™ U21654
Additional Details : Weight : 0.00450kg
Description
ULYSIS™ Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast—just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.ULYSIS™ Nucleic Acid Labeling Specifications:
The Resulting Labeled Probe is useful for:
Reliable Labeling With the Universal Linkage System
We developed this series of ULYSIS™ kits to enable rapid and simple coupling of our Alexa Fluor™ dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS™), is based on the use of a platinum dye complex (owned by KREATECH Diagnostics) that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.
Labeling is Fast and Easy
The labeling reaction typically takes only 15 minutes, and separation of the labeled nucleic acids from the unreacted ULS™ complex can be accomplished through the use of a simple spin-column procedure. DNA longer than ∼1,000 base pairs requires a 10-minute DNase digestion before labeling, which both optimizes labeling and fragments the probe for efficient hybridization.
More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling, review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook, read Nucleic Acid Amplification & Expression Profiling, or view a list of our kits.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Specifications
| Store in freezer (-5 to -30°C) and protect from light. | |
| Kit | |
| Probes (Labeled RNA), Probes (Labeled DNA), Oligos (Labeled) | |
| Yes | |
| Alexa Fluor™, Ulysis™ | |
| Room Temperature |
| Fluorescence | |
| Alexa Fluor 594 | |
| DNA (General), Oligos, RNA (General) | |
| Direct Labeling | |
| Nucleic Acid Labeling Kit |
For Research Use Only. Not for use in diagnostic procedures.