Nucleic Acid Labeling and Detection
Nucleic Acid Labeling and Detection
Nucleic acid labeling and detection products are used to add tags or labels to nucleic acids to enable detection or purification. The tagged nucleic acids are referred to as 'probes.'
Nucleotide labeling may include radioactive phosphates, biotin, fluorophores, and various enzymes. Bioconjugation methods that produce nucleic acid probes can also be adapted to attach them to other molecules (for targeted delivery) and surfaces (to facilitate immobilization).
Although nucleic acid probes can be labeled during synthesis, custom oligonucleotide probes can be expensive so many researchers choose to label their own.
Quick and efficient benchtop oligonucleotide labeling can be performed for making smaller probe amounts or when multiple probes with the same label are needed. Enzymatic methods are affordable for small-scale probe generation; chemical methods are more suited to larger scale reactions.
Probes can be labeled at either end of the oligonucleotide or randomly incorporated throughout the sequence. The choice of method depends on the degree of labeling required and whether changes will prevent theexpected interactions. Nucleic acid hybridization reactions can benefit from random label incorporation; protein interactions may require end-labelling.
Summary of Methods
Enzymatic
- TdT: ssDNA
- T4 RNA Ligase: ssDNA, RNA
- T4 PNK: ssDNA, RNA
- DNA Polymerase: DNA, RNA
- RNA Polymerase: RNA
Chemical
- Periodate: RNA
- EDC: DNA, RNA
- Nonspecific crosslinkers: DNA, RNA
Detection methods are biotin-based, chemiluminescent, fluorescent, radioactive, or the combination of several techniques. Tests may include nucleic acid tests for pathogen detection and fluorescent in situ hybridization (FISH) for specific DNA sequences in chromosomes.
Thermo Scientific™ Pierce™ RNA 3' End Biotinylation Kit
Biotinylate 3-prime terminal ends of RNA probes for non-radioactive EMSA and other nucleic acid detection methods.
Molecular Probes™ ARES™ Alexa Fluor™ 488 DNA Labeling Kit
ARES™ Alexa Fluor™ 488 DNA Labeling Kit
Thermo Scientific™ Nucleic Acid Detection Blocking Buffer, 500mL
Nucleic Acid Detection Blocking Buffer is intended for use with the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module Kit
Invitrogen™ TE Buffer (20X), RNase-free
Essential to success of RiboGreen™ RNA quantitation assays
Promotions are available
Invitrogen™ Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit
Detect newly synthesized RNA or changes in RNA levels
Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit
Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.
Invitrogen™ FluoReporter™ Blue Fluorometric dsDNA Quantitation Kit, 200-2,000 assays
Provide rotocols and reagents for analyzing cellular NA with blue-fluorescent Hoechst 33258 nucleic acid stain
Thermo Scientific™ North2South™ Biotin Random Prime DNA Labeling Kit
Produce biotinylated DNA templates for DNA hybridization and detection methods using random heptanucleotides, Klenow fragment and biotin-nucleotides.
Thermo Scientific™ DecaLabel DNA Labeling Kit
Thermo Scientific™ Fermentas DecaLabel DNA Labeling Kit is a complete system for fast synthesis of radiolabeled DNA probes of high specific activity.
Molecular Probes™ FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye
FISH Tag™ DNA Orange Kit, with Alexa Fluor™ 555 dye
Molecular Probes™ FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination
FISH Tag™ DNA Multicolor Kit, Alexa Fluor™ dye combination
Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)
For easy and accurate dsDNA quantitation, the Quant-iT 1X dsDNA HS and BR Assays enable up to 1,000 fluorescence-based assays in a 96-well microplate format.
Invitrogen™ 100X Pretreatment Solution
100X Pretreatment Solution is an aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.
Invitrogen™ AP Enhancer Solution
AP Enhancer Solution, 10 mL is an aqueous buffered solution and an individual component of the ViewRNA™ ISH Tissue Assay Kits, 2-Plex, 24 assays.
Invitrogen™ PrimeFlow™ RNA Assay Kit
An in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the detection of four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies.
Invitrogen™ Qubit™ 1X dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
Invitrogen™ Quant-it™ RiboGreen RNA Assay Kit and RiboGreen RNA Reagent, RediPlate™ 96 RiboGreen™ RNA Quantitation Kit
Rapid, simple, and highly sensitive fluorescence-based RNA-quantitation assays are enabled with the RediPlate 96 RiboGreen RNA Quantitation Kit. The microplate is pre-loaded with RiboGreen Reagent, which produces bright green fluorescence upon binding to RNA or DNA.
Molecular Probes™ FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination
FISH Tag™ RNA Multicolor Kit, Alexa Fluor™ dye combination
Molecular Probes™ Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit
Alexa Fluor™ 488 Oligonucleotide Amine Labeling Kit
Invitrogen™ Qubit™ dsDNA HS and BR Assay Kits
For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/uL to 100 ng/uL, and 100 pg/uL to 1,000 ng/uL, respectively.
Molecular Probes™ Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
Ulysis™ Alexa Fluor™ 594 Nucleic Acid Labeling Kit
Thermo Scientific™ North2South™ Hybridization Buffer
Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.
Thermo Scientific™ Chemiluminescent Nucleic Acid Detection Module Kit
Detect biotin-labeled nucleic acid probes in various membrane blotting applications with this complete chemiluminescent detection system.
Invitrogen™ Silencer™ siRNA Labeling Kit with FAM dye
Silencer™ siRNA Labeling Kit with FAM dye
Thermo Scientific™ LightShift™ EMSA Optimization and Control Kit
Identify and characterize protein-DNA binding interactions by EMSA with this extraordinarily robust and sensitive system.
Invitrogen™ Click-iT™ Nascent RNA Capture Kit, for gene expression analysis
Perfect for researchers in academia and industrial settings
Invitrogen™ Qubit™ ssDNA Assay Kit
Quantitate oligonucleotides or long ssDNA easily and quickly with the Qubit ssDNA Assay Kit, which works with the Qubit Fluorometer and other fluorometers or plate readers. Common contaminants such as salts, solvents, detergents, and protein are well tolerated with this assay.
Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit
Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.
Invitrogen™ Qubit™ RNA IQ Assay Kits
Obtain ready-to-use detection of RNA degradation information with the Qubit RNA integrity and quality (IQ) Assay Kit and Standards, which feature two unique dyes for binding large and/or highly structured RNA and small, degraded RNA. Together, these dyes enable detection of RNA quality and integrity.