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Use amplified DNA directly for cycle sequencing without purification. Cytiva illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA.
Includes | 0.5mL Sample Buffer, 0.5mL Reaction Buffer, 20ÎĽL Enzyme mix and 50ÎĽL positive control pUC19 DNA |
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Content And Storage | The product can be stored for 2 months or less at -20°C. For storage longer than 2 months, store at -70°CThaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior to amplification |
Description | illustra TempliPhi DNA Amplification Kits efficiently prepare DNA sequencing templates from circular DNA. |
For Use With (Application) | Sequencing Template Amplification Kits and Reagents |
Test Time | 4-18 h depending on starting material |
Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications
Uses modified nucleotides in isothermal rolling circle amplification (RCA) to improve results with difficult to sequence templates
Content And Storage | Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°CThaw components on ice and maintain at 0°C to 4°C during handling |
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Description | illustra TempliPhi Sequence Resolver Kit uses the highly processive, strand-displacing Phi29 DNA polymerase and modified nucleotides to amplify circular templates for subsequent sequencing. |
Product Type | illustra TempliPhi Sequence Resolver Kit |
For Use With (Application) | Amplification of difficult templates for successful DNA sequencing |
Test Time | 18 h |