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Invitrogen™ RNAsecure™ RNase Inactivation Reagent

Invitrogen™ RNAsecure™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution.

738.00 SEK - 3120.00 SEK

Specifications

Content And Storage Store RNAsecure™ at -20°C.
For Use With (Application) RT-PCR, In Vitro Transcription
Product Line Ambion™, RNAsecure
Product Type RNase Control Reagent
Purity Molecular Biology Grade
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10229254
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Invitrogen™
AM7006
10 mL
3120.00 SEK
Each
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10066044
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Invitrogen™
AM7005
1 mL
738.00 SEK
Each
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Description

Description

Invitrogen™ RNAsecure™ (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution.

• No post-treatment autoclaving
• Safe, easy-to-use reagent inactivates RNases in solutions
• Inactivation process can be repeated to protect against newly introduced contaminants
• Compatible with downstream procedures, such as RT-PCR and in vitro transcription

Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and, at worst, can cause experimental failure.

Traditionally, DEPC has been used to treat solutions that come into contact with RNA. DEPC treatment is time-consuming, possibly hazardous, and only eliminates RNases present at the time of DEPC treatment. In addition, some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. Finally, DEPC has to be inactivated by autoclaving post-treatment to prevent it from interfering with downstream enzymatic reactions.

RNAsecure™ Reagent eliminates these problems. Unlike DEPC, RNAsecure™ can be used on virtually any solution and does not require post-treatment autoclaving.

A unique feature of RNAsecure™ is that reheating after the initial treatment will reactivate the RNase-destroying agent to eliminate any new contaminants. RNAsecure™ is supplied as a 25X concentrated stock. It is added to solutions as part of reactions (in vitro transcription, RT-PCR, etc.), prior to the addition of enzymes, and heated to 60°C for 10 min to inactivate RNase.

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Store RNAsecure™ at -20°C.
Ambion™, RNAsecure
Molecular Biology Grade
Liquid
RT-PCR, In Vitro Transcription
RNase Control Reagent
Dry Ice
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.