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CD19 Mouse anti-Human, Clone: HIB19, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0199-82

Additional Details : Weight : 0.01000kg

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Product Code. 15216847

  • 886.00 SEK / 100µg

In stock, ready to ship: 1

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Description

Description

Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in in vitro functional studies. (Please use Functional Grade Purified HIB19, cat. 16-0199, in functional assays. Fluorochrome-conjugated HIB19 is recommended for use in flow cytometry.). Applications Tested: This HIB19 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and maligt B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Specifications

Specifications

CD19
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P15391
AW495831, B-lymphocyte antigen CD19, differentiation antigen CD19
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen)
HIB19
Unconjugated
CD19
Liquid
CD19
IgG1, kappa
100 μg
4° C
Primary
930
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For Research Use Only.