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  6. Thermo Scientific™ GeneJET Plasmid Miniprep Kit
  7. 12793184

Thermo Scientific™ GeneJET Plasmid Miniprep Kit

Perform isolation of high quality plasmid DNA from recombinant E. coli cultures with this simple, rapid, and cost-effective system.

Brand:  Thermo Scientific™ K0502

Additional Details : Weight : 0.41000kg Transport : UN number : 1824 Chem class : 8 Pack group : III


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Product Code. 12793184

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Description

Description

The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20μg of high copy plasmid DNA per isolation procedure.

  • Efficient—high yields of up to 20μg of high quality plasmid DNA

  • Fast—procedure takes less than 14 min

  • Convenient—no phenol/chloroform extraction or alcohol precipitation required

  • Pure—purified DNA is immediately ready to use


Applications

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:

  • FastDigest or conventional restriction digestion
  • Automated fluorescent and radioactive sequencing
  • PCR
  • In vitro transcription
  • Transformation


Principle

A bacterial culture is harvested and lysed. The lysate is then cleared by centrifugation and applied on the silica column to selectively bind DNA molecules at a high salt concentration. The adsorbed DNA is washed to remove contaminants, and the pure plasmid DNA is eluted in a small volume of elution buffer or water. The purified DNA is ready for immediate use in all molecular biology procedures, such as fast and conventional digestion with restriction enzymes, PCR, in vitro transcription, transformation, and automated sequencing. 

Recommended for:

Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:—FastDigest or conventional restriction digestion,—automated fluorescent and radioactive sequencing

Specifications

Specifications

PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription
Bacteria
Compatible (Vacuum manifold)
GeneJET
Plasmid DNA
50 preps
< 100 µg (Small-Scale) Plasmid DNA
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Safety and Handling

Safety and Handling

Product Identifier
  • GeneJET Plasmid Miniprep Kit

Signal Word
  • Danger

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Acute toxicity Category 4

Hazard Statement
  • H319-Causes serious eye irritation.
  • H315-Causes skin irritation.
  • H302-Harmful if swallowed.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P302+P352-IF ON SKIN: Wash with plenty of water/.
  • P264-Wash thoroughly after handling.
  • P373-DO NOT fight fire when fire reaches explosives.

Supplemental information
  • MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,Guanidine hydrochloride

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